The endophytic microbiome in orchid plants is richdiverse; however, few studies have analyzed the endophytic microbiome of Cymbidium plants in diferent tissuessoils. This study implemented the Illumina Miseq technology to investigate the diversity of endophytic fungi in diferent tissues of wild Cymbidium goeringii. The results demonstrated that diferent tissue samples harbor a rich fungal endophytic community,those fungi could be classifed4 phyla, at least 145 families,185 genera. The endophytic fungal community diversity difered among the orchid tissuessoils,some fungal taxa were clearly concentrated in certain orchid tissues, with more operational taxonomic units (OTUs) being detected. Investigation of mycorrhizal associations showed that 43 (about 3.8%) of the total 1137 OTUs could be assigned as Orchidaceae mycorrhizal fungi (OMF), while about 96.2% the OTUs were non-mycorrhizal fungi. Among the OMFs, OTUs of the ectomycorrhizal fungi RussulaceaeThelephoraceae families were the most abundant, with diferent richness in the soil, followed by TulasnellaceaeCeratobasidiaceae, which were dominant in the root communities of C. goeringii.In the seeds, the absolutely dominant family was Nectriaceae,the common OMFs Ceratobasidiaceae (fve OTUs)Tulasnellaceae (one OTU) were also detected in the seeds. Two Tulasnella spp. isolates from the roots of wild C. goeringii could efectively promote seed germinationrhizome formation of wild C. goeringii,these strains might be particularly important in the practice of conservation for many endangered C. goeringii in China.